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Image Search Results
Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: Carotid Baroreceptor Stimulation Ameliorates Pulmonary Arterial Remodeling in Rats With Hypoxia‐Induced Pulmonary Hypertension
doi: 10.1161/JAHA.124.035868
Figure Lengend Snippet: A and B , Representative photomicrographs of RV with H&E staining ( A ) and Masson staining ( B ). Scale bar=20 μm. n=5. C , Bar graph showed the size of RV cardiomyocyte CSA. n=5. D , Weight ratio of RV to LV+S. n=5. E , Weight ratio of RV to BW. n=5. F , RVWT assessed by echocardiographic measurements. n=7–10. G , real‐time quantitative polymerase chain reaction analysis showed the relative mRNA expression level of ANP in RV. n=3. H , The result of quantitative analysis of CVF in RV. n=5. I and J , Representative immunoblot bands and the protein expression level of MMP2 and MMP9. n=3. Values were mean±SEM. * P <0.05, ** P <0.01, *** P <0.001. ANP indicates atrial natriuretic peptide; CBS, carotid baroreceptor stimulation; Con, Control; CSA, cross‐sectional area; CVF, collagen volume fraction; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; H&E, hematoxylin and eosin; HPH, hypoxia‐induced pulmonary hypertension; MMP2, matrix metallopeptidase 2; MMP9, matrix metallopeptidase 9; RV, right ventricle; RV/BW, weight ratio of RV to body weight; RV/LV+S, weight ratio of RV to left ventricle plus septum; and RVWT, RV wall thickness.
Article Snippet: The cDNA library preparation was conducted using an
Techniques: Staining, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Control
Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: Carotid Baroreceptor Stimulation Ameliorates Pulmonary Arterial Remodeling in Rats With Hypoxia‐Induced Pulmonary Hypertension
doi: 10.1161/JAHA.124.035868
Figure Lengend Snippet: A , Venn map showed DEGs among Con‐sham, Hypoxia‐sham and Hypoxia‐CBS group. B , CBS made a change in DEGs altered by hypoxia, which was shown in GO enrichment analysis. C , PPI network showed that the top hub genes of DEGs. D , Heat map showed the 21 hypoxia‐upregulated genes reversed by CBS treatment. E and F , The relative mRNA expression levels of POSTN ( E ) and BDNF ( F ) Con‐sham, n=3; Con‐CBS, n=3; Hypoxia‐sham, n=4; Hypoxia‐CBS, n=4 (normalized to GAPDH mRNA). Values were mean±SEM. * P <0.05, ** P <0.01, *** P <0.001. BDNF indicates brain‐derived neurotrophic factor; BP, biological process; CBS, carotid baroreceptor stimulation; CC, cellular component; Con, Control; DEGs, differentially expressed genes; GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; GO, Gene Ontology; MF, molecular function; POSTN, periostin; and PPI, protein–protein interaction network.
Article Snippet: The cDNA library preparation was conducted using an
Techniques: Expressing, Derivative Assay, Control